MichaelÌýNagel
Mechanisms of transcription re-initiation
Transcription reinitiation occurs following a pioneering round of transcription but remains poorly understood. Reinitiation events produce most RNA transcripts and are essential for robust biological responses.ÌýÌýReinitiation may occur through mechanisms distinct from "first-round" transcription initiation.ÌýÌýA reinitiation scaffold, discovered inÌýS. cerevisiaeÌýby the Steve Hahn lab, consists of a set of general transcription factors that remain promoter-associated following the first round of transcription; this scaffold may similarly operate at human genes, to facilitate transcription re-initiation.ÌýÌýMy project implements biochemical and cell-based methods to characterize theÌýmolecular mechanisms that control transcription re-initiation in human cells.Ìý
Publications
Zhou, W., E. Ramos, X. Zhu, P. M. Fisher, M. E. Kidane, B. H. Vanderloop, C. D. Thomas, J. Yan, U. Singha, M. Chaudhuri, M. T. Nagel, and W. D. Nes. Steroidal antibiotics are antimetabolites of Acanthamoeba steroidogenesis with phylogenetic implications.ÌýJÌýLipid ResÌý2019, 60: 981–994.